Yes, you would need to shorten the barrel extension and redo the forcing cone. You would also have to adjust the frame lug that holds the cylinder from moving to the rear when open and ejecting. 45 colt cylinders are longer in both directions.

But, finding a 5" 45 colt with a round butt frame isn't very likely. Reason I make my own is 45 colts are hard to find. I have made 2 of them for 629 frames and another from an 45 acp frame. I have the parts to do another after I mod this 25-5 and am going to use a 28-2 frame.