It depends on the revolver. If the charge holes are bored straight through, like Iver Johnson guns were, and the loaded cartridge isn't longer than the cylinder, then yes. The other caveat is you must understand loading with Black Powder. The charge MUST fill the to the point it is slightly compressed by the bullet. It is imperative that there be no free airspace within the loaded case or the gun can be damaged.
If the revolver in question has normal charge holes, with a bullet diameter throat ahead of the larger portion for the case, then no. This would include all higher quality guns.
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Gunsmithing since 1961
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