Yes. You cannot put an 8 shot cylinder in a 6 shot frame, or a 6 shot cylinder in an 8 shot frame without dire consequences. The reason is that the 8 shot cylinder bolt circle (centerline of chambers) is .06 larger diameter, or .03 larger radius. That means that the 8 shot chamber would be vertically misaligned to the barrel by .03 in a 6 shot frame. If you fired the gun with that much misalignment, it would probably blow it up. I say probably because I'm not going to try it to find out.