Troy,
The #5401 is the arsenal rebuid # I mentioned in post #11.
There are 6 matching serial # locations for fixed sight pre war Hand Ejectors and all Hand Ejectors until ~1956.
NOTE: Observing serial #s for accuracy or even existence, especially on penciled stocks, requires magnification, bright light, and an attitude that it is there!
1. Gun butt - or forestrap on I frames/single shots with grips that cover the butt
2. Barrel - bottom of barrel or in extractor shroud
3. Yoke - on rear face only visible thru a chamber with a flashlight
4. Extractor star - backside
5. Cylinder - rear face
6. Right stock only - on back; stamped, scratched or penciled depending on vintage and stock material. (except most target grips because individual fitting not required.)
Assembly (factory work) #s: These multi-digit numbers of 3 to 5 digits, are on the yoke at the hinge, in the 'yoke cut' on frame opposite the yoke near the hinge, and inside of the sideplate, for the pre war and early post war period. The assembly # in the yoke cut of the frame was relocated to the left side of grip frame after model #s were assigned and the serial # was added in the 'yoke cut' where the assembly #, now moved to the left side of the grip frame, used to be. You know they are assembly (factory work) #s because of those 3 locations that always match on guns that are original, and that's the only usefulness for them after guns leave the factory: still used to this day, long after serial number locations decreased.