Check the pin ("locking bolt") at the front of the barrel lug. With the gun assembled, as you close the yoke slowly, you should see that pin protrude/walk out toward the muzzle, and then pop back in as everything closes.
If it protrudes and stays there, you might have a bent yoke. That's the only reason I can think of for the center pin to not seat as it should, in light of the fact that it seats with the yoke removed. Examine the movement of the locking bolt carefully as you open and close the cylinder.
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I just watched your video a second time. I think I'm right. If you look at about the 1:48 mark and at times afterward, you'll see that the locking bolt is protruding more than it should. It's quite clear.
Either your ejector rod is bent and you're positioning it the same way every time you close the action, which is extremely unlikely, or far more likely your yoke is bent.
To fix it, you'll need a "yoke alignment tool" available from several sources, and a small hammer with a plastic face, or some suitable tool to persuade the yoke into proper alignment.