I'm assuming you are experiencing the resistance to cylinder rotation with all 6 chambers loaded even when trying to rotate the cylinder by hand? That is, rotating by hand while the cylinder stop is dropped down. To check, you can put a business card or piece of paper between the cylinder and the frame over the cyl stop to render the stop inoperable, then try to turn the cylinder. If there is resistance when turning the cylinder by hand when it is closed, this would rule out some sort of problem with the hand ratchet interface.
Also, try removing the yoke, degreasing the bearing surface at the end (back) of the yoke barrel, apply a coating of Dykem or "Sharpie" marker to the bearing surface.....then re-install the cylinder/yoke in the gun and dry fire the gun several times with snap caps in the four chambers that are ok, leaving the offending chambers empty. After dry firing, remove the yoke and look at the previously applied coating on the yoke bearing surface (end) with a magnifier. Is the applied coating rubbed off all the way around the end of the bearing surface, or is there a shiny spot? A shiny spot on the surface with coating remaining elsewhere would indicate a "high spot" that may be affecting the normal rotation of the cylinder.
If you have binding on the yoke determined above.....check your front gauge with the cylinder closed and the cylinder assy pushed forward. If you have room in the front gauge....over .004", you can remove a small amount of material from the "high spot" on the yoke bearing area at the end of the yoke barrel. The idea here would be to make the bearing surface at the end of the yoke barrel 90° to the bearing surface inside the cylinder. You will have to re-test and remove material by hand (stone) unless you have a yoke facing cutter. Remember, you will only need to remove a small amount of material to eliminate the high spot found....if there is one. (probably .001" or thereabouts, no more)
Creating a bit more necessary gauge or space in the cylinder assy may free up your rotation issue. Basically, you are adding in just a bit of necessary "end shake" into that system. It won't work (rotate, open/close) properly without this space. Lubricant on that yoke is also essential. A small amount of light oil on the yoke barrel is all that is needed.
Yoke alignment to the center pin hole in the frame is also a consideration here, as is severe runout on the extractor rod. Make sure the center pin is in tact and straight.....and that the yoke is aligned with the fame. Yoke alignment tools and gauges are available. Make sure the extractor rod is straight, with as little runout as possible. Runout here can cause interference by the front locking bolt.
POWER CUSTOM EXTRACTOR ROD & YOKE ALIGNMENT TOOL | Brownells