Keith, the vertical stringing is most likely the usual culprit - in other words, difficulty in resolving the perfect match of front and rear sight in elevation. I always have trouble with this in bench shooting a revolver and changed to prone years ago when I discovered that somehow prone worked better.
I would encourage you to try the biggest bullet you can stuff in that new cylinder. If a .453 bullet can be pushed through I would try a .454. If the loaded round will drop into the chambers I think it's no risk and might serve to get you a little more accuracy.
I'm glad things have worked out for you with this gun. Staying on the project paid off, didn't it?