I EDC a 642 and do a daily function check to make sure all the screws and nuts are tight.
Clean and degrease the threads on the nut and make sure it's tightened snugly.
These steps were not enough to keep the cylinder latch nut on my gun from loosening, so I did end up using a small amount of blue loctite (purple would likely be better). However, I used a very small amount and did what I could to keep it from possibly wicking down into the action.
What I did was put a drop of loctite on a paper towel. I took a toothpick and just touched the tip of it to the drop. I used the toothpick to apply it to the threads inside the nut, NOT on the bolt (putting it on the bolt might increase the chances of loctite getting into the action, as slim as that chance might be). I then tightened the nut snugly and laid the gun down on it's left side, with the cylinder release latch on the bottom, again to prevent loctite from getting into the action. I left it overnight to make sure it fully cured. A function test in the morning showed all was well. I did this nearly two years ago and daily carry, regular dry fire practice, and live fire range visits have not caused it to loosen at all in that time and I have not experienced any function/reliability issues.
Try to avoid using loctite if you can, but if you must then I think the procedure I used will go a long way towards preventing any problems.